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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Inhibitory ITAM signaling by Fc alpha RI-FcR gamma chain controls multiple activating responses and prevents renal inflammation.
doi: 10.4049/jimmunol.180.4.2669
Figure Lengend Snippet: FIGURE 3. Treatment of FcRI transgenic mice with anti-FcRI Fab A77 improves renal disease parameters in anti-GBM-induced glomerulonephritis. A, Evaluation of proteinuria over 14 days in mice after induction of anti-GBM glomerulonephritis and treatment with PBS, Fab A77, or Fab 320 as described in Materials and Methods. B, Evaluation of blood urea nitrogen (BUN) and creatinine levels 7 and 14 days after induction of anti-GBM glomerulonephritis in mice treated with PBS, Fab A77, or Fab 320. C, Histologic analysis (PAS staining) after induction of anti-GBM glomerulonephritis and following treatment with PBS, Fab A77, or Fab 320. Corresponding quantitative evaluation (below) of histologic disease parameters by disease severity score is shown. , p 0.05. D, Immunohistological analysis of CD4-, Mac-1-, and F4/80-positive cells following treatment with PBS, Fab A77, or Fab 320, and corresponding quantitative analysis (below) of intraglomerular and periglomerular infiltrating cells per glomerular cross-sections (gcs) is shown below the staining. , p 0.05.
Article Snippet: The following Abs were also used: rabbit monoclonal anti-CCR2 mAb (Epitomics), rat antitype I collagen (Southern Biotechnology Associates), rat anti-Mac1 (Serotec), rabbit anti-phospho-p38 or anti-phospho-ERK MAPK Abs (Cell Signaling Technology), rabbit anti-phospho-JNK and rabbit anti-SHP-1 (Santa Cruz Biotechnology), mouse anti- -actin mAb (Sigma-Aldrich) and
Techniques: Transgenic Assay, Staining
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Inhibitory ITAM signaling by Fc alpha RI-FcR gamma chain controls multiple activating responses and prevents renal inflammation.
doi: 10.4049/jimmunol.180.4.2669
Figure Lengend Snippet: FIGURE 5. Treatment of FcRI transgenic (Tg) mice with anti-FcRI Fab A77 inhibits inflammatory cell infiltration after UUO. A, Immunohistological analysis of Mac-1-, F4/80-, and CD4-positive cells in FcRI transgenic mice after UUO (day 14) following treatment with either PBS, Fab A77, or control Fab 320, as described in Materials and Methods. The corresponding quantitative analysis of infiltrating cells. , p 0.05. B, Immunohistological analysis of Mac-1-, F4/80-, and CD4-positive cells in FcRIR209L transgenic mice after UUO (day 14), following treatment with PBS, Fab A77, or control Fab 320. Corresponding quantitative analysis of infiltrating cells per high-power field (HPF) is shown below each staining.
Article Snippet: The following Abs were also used: rabbit monoclonal anti-CCR2 mAb (Epitomics), rat antitype I collagen (Southern Biotechnology Associates), rat anti-Mac1 (Serotec), rabbit anti-phospho-p38 or anti-phospho-ERK MAPK Abs (Cell Signaling Technology), rabbit anti-phospho-JNK and rabbit anti-SHP-1 (Santa Cruz Biotechnology), mouse anti- -actin mAb (Sigma-Aldrich) and
Techniques: Transgenic Assay, Control, Staining